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. 2021 Jul 8;12:692127. doi: 10.3389/fimmu.2021.692127

Figure 4.

Figure 4

TL1A induces mucus production independently of adaptive immunity, bystander to IL13 production by ILC2. (A) Schematic representation of the protocol used, as previously described in Figure 1 . (B) Top panel: PAS stain of mucus produced in the lungs of RAG-/- and RAGγc-/- mice induced with PBS or TL1A and quantified using Image Pro Premier (right graph). Lower panels: Immunofluorescence stains of MUC5AC (red) and nuclear DAPI (blue). (C) DR3 staining on ILC2 assessed by flow cytometry (DR3 in blue and Isotype in red). (D) Number of lung ILC2 in the left lobe of RAG-/- mice induced with TL1A. (E) IL13 levels assessed by ELISA, in the supernatants of ILC2 cultured in the presence of IL2, IL7, and IL33 and stimulated with or without TL1A. All results representative of three experiments with four to six mice per group, or three individual replicate cultures. *p < 0.05, **p < 0.005.