Fig. 1 |. γδ T cells are enriched and resident in adipose tissue.

a, Representative flow cytometry plots of γδ T cells from the epididymal WAT stromal vascular fraction (eWAT SVF). K denotes thousand. b, Frequency of γδ T cells across various adipose tissue depots as a percentage of CD3ε⁺ T cells in male eWAT (n ≥ 4 mice per group). Each symbol represents an individual mouse; small horizontal lines indicate the mean. c, Flow cytometry of lymphocytes in the blood, liver, spleen, and adipose tissue of CD45.1⁺ and CD45.2⁺ congenic C57BL/6 parabiotic pairs joined at 6 weeks of age and analyzed 2 weeks later (left); and frequency pie charts of CD45.1⁺ and CD45.2⁺ CD8⁺ and γδ T cells (right). d, Frequency of Vδ1⁺, Vδ2⁺, and Vδ3⁺ γδ T cells (% of CD45⁺PI⁻ cells) in peripheral blood (PBMCs) or from matched omental fat from patients before bariatric surgery. Data are representative of three experiments (a,b,d; mean ± s.e.m. in b) or one experiment (c).