TABLE 5.
Identified mutations in CH34-TIMMR1 (R1) and CH34-TIMMR2 (R2).
| Replicon1 | Gene2 | Protein function | Mutation3 | R1 | R2 |
| CHR1 | hox/cbb | Hydrogenotrophy | Δ 60 kb | + | + |
| CHR1 | 0862 | Conserved hypothetical protein | INS IS1088 | + | − |
| CHR1 | 1009 | Oxidoreductase/nitroreductase | SNP | + | + |
| CHR1 | 1107 | Putative membrane protein | INS IS1088 | + | − |
| CHR1 | 1492 | Fructose-bisphosphate aldolase | 2 SNPs | + | − |
| CHR1 | 1563 | Transposase ISRme12 | 2 SNPs | + | − |
| CHR1 | 1885 | Proteolytic subunit of ClpA-ClpP and ClpX-ClpP ATP-dependent serine proteases | SNP | − | + |
| CHR1 | 1886 | Peptidyl-prolyl cis/trans isomerase | INS IS1090 | − | + |
| CHR1 | 2409 | Phosphatidylglyc erophosphate synthetase | INS IS1088 | + | − |
| CHR1 | 6452 | Hypothetical protein | 2 SNPs | − | + |
| CHR2 | 3658 | Type II secretion system protein | SNP | − | + |
| CHR2 | 4106 | Acetone carboxylase α-subunit | SNP | + | − |
| CHR2 | 4757 | Hypothetical protein | INS Tn6048 | − | + |
| CHR2 | 4970 | Formyl-CoA transferase | SNP | − | + |
| CHR2 | 4972 | Tn6048 | Δ 6 kb | − | + |
| CHR2 | 5469 | Hypothetical protein | INS IS1086 | + | − |
| CHR2 | 5806 | Ferric citrate outer membrane transporter | INS IS1088 | + | − |
| pMOL28 | 6321 | Conserved hypothetical protein | +1 frameshift | + | + |
| pMOL28 | 6326 | Conserved hypothetical protein | INS IS1087B | + | − |
| pMOL30 | 6072 | Putative transcriptional regulator | SNPs | + | − |
| pMOL30 | 6072 | Putative transcriptional regulator | INS ISRme15 | + | − |
| pMOL30 | 6097 | 2-Nitropropane dioxygenase | SNP | − | + |
1CHR1: chromosome; CHR2: chromid, pMOL28 and pMOL30 are megaplasmids. 2Locus tag (Rmet_XXXX). 3Δ: deletion, INS: insertion, SNP: single-nucleotide polymorphism.