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. Author manuscript; available in PMC: 2021 Jul 23.
Published in final edited form as: Cell Rep. 2021 Mar 23;34(12):108891. doi: 10.1016/j.celrep.2021.108891

Figure 2. Pro-inflammatory cytokine and chemokine genes are highly induced in response to TLR7/8 agonist (R848) in monocytes.

Figure 2.

(A) Heatmap showing the expression of genes that are significantly upregulated by R848 stimulation in monocytes.

(B) Summary table showing Ingenuity Pathway Analysis networks or canonical pathways of genes and signature genes in cluster VII.

(C) IL-1β (left) and IL-6 (right) production by monocytes, MDMs, and MDDCs from 5 donors measured by ELISA following TLR stimulation. Monocytes, MDMs, and MDDCs were unstimulated or stimulated with Pam3CSK4 (1 μg/mL), LPS (10 ng/mL), R837 (10 μg/mL), or R848 (10 μg/mL) for 18 h. Data represent the mean values derived from 2 independent experiments with conditions performed in duplicate. Error bars indicate SEMs. Statistics was determined by ordinary 2-way ANOVA. ****p < 0.0001.

See also Figure S3 and Table S3.