Figure 5.

Representative immunofluorescent staining images showing the differential expression patterns of six cell cycle-dependent genes at the protein level in HLECs. (A) Six antibodies (green) stained more strongly and with greater frequency in small cells than larger cells, with Ki67 as control. DAPI (blue) was used for nuclear counterstaining. Yellow circle: small cells; red circle: large cells. (B–D) Protein distribution patterns of seven TAC markers. Ki67 showed nuclear staining, and RRM2 and TK1 were entirely cytoplasmic (B). CENPF was partially cytoplasmic surrounding the nucleus and often positive in the dividing cells. NUSAP1 was located at chromosomes in the nucleus; the figure shows the different stages of spindle formation (C). UBE2C and CDC20 were mainly located in the cytoplasm of dividing cells (D). (E) BrdU incorporation with TAC markers. RRM2 and TK1 were partially co-stained with BrdU, but NUSAP1 and CDC20 did not co-stain with BrdU.