Figure 8.

Effects of sCD146 and vascular endothelial growth factor (VEGF) on the migration of human retinal microvascular endothelial cells (HRMECs). Overnight starved HRMECs were left untreated or treated either with sCD146 (100 ng/ml) or with VEGF (50 ng/ml) for 18 h. Cells were visualized using an inverted microscope. Two independent experiments were performed. Each experiment was done in duplicate and 6-8 independent field images were taken for the migration analysis which was done by using Image J software. In the figure, one representative image is illustrated, and the bar graphs show the analysis of all the images from each group represented as fold change in migration versus control. Results are expressed as mean ±SD (*p < 0.05; independent t-test).