Figure 1.

RGC-NSC direct cocultures promote RGC process outgrowth. Representative images of (A) GFP-positive RGCs cultured in isolation under standard conditions or (B) GFP-positive RGCs directly cocultured with tdTomato-expressing NSCs. (C) After two days in culture, an increase in the longest RGC process length was observed in RGCs directly cocultured with NSCs. (D) RGC-NSC direct cocultures did not change the percentage of RGCs with processes. *P < 0.05; Student's t-test. N = 4 experiments; n > 1000 cells/experiment; error bars represent SEM. Scale bars: 100 µm.