Figure 2.

NSC-conditioned media promotes RGC survival and neurite outgrowth. Representative images of calcein AM-stained RGCs cultured in (A) standard RGC media or (B) 10% NSC-conditioned RGC media. RGCs cultured with BDNF and CNTF or increasing concentrations of NSC-conditioned media (NCM) demonstrated dose-dependent (C) increases in neurite outgrowth and (D) increases in the percentage of RGCs with neurite. (E) RGCs cultured with BDNF or increasing concentrations of NCM showed increase rates of survival. *P < 0.05; one-way ANOVA with post-hoc Tukey test. N = 4 experiments; n > 1000 cells/experiment; error bars represent SEM. Scale bars: 100 µm.