Table 2.
Antimicrobial activity of ZnNPs and CurNPs against the tested bacteria and fungi represented by the inhibition zones diameters (mm), MIC, MFC and MBC (µg/mL). (n = 6).
| Microorganisms | ZnNPs (µg/mL) | Means | CurNPs (µg/mL) | Means | MIC | MBC | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Bacteria | 100 | 150 | 200 | 250 | 300 | 100 | 150 | 200 | 250 | 300 | ZnNPs | CurNPs | ZnNPs | CurNPs | ||
| B. cereus | 17.26 b | 18.22 b | 20.91 b | 21.65 b | 22.84 b | 20.18 AB | 18.32 b | 19.62 b | 21.41 b | 22.22 b | 23.43 b | 21.00 AB | 60.12 d | 55.23 d | 110.00 e | 100.31 e |
| L. monocytogenes | 16.55 c | 17.53 c | 19.52 c | 20.54 c | 23.42 c | 19.5 B | 17.43 c | 18.93 c | 20.95 c | 23.43 c | 24.22 c | 20.99 B | 75.21 cd | 69.24 cd | 130.01 d | 120.21 d |
| S. pyogenes | 18.36 a | 19.45 a | 21.83 a | 22.92 a | 24.53 a | 21.42 A | 19.55 a | 20.34 a | 22.53 a | 23.65 a | 25.64 a | 22.34 A | 50.00 e | 45.33 e | 90.23 f | 85.43 f |
| E. coli | 15.28 d | 16.96 d | 18.81 d | 19.57 d | 20.67 d | 18.26 BC | 16.28 d | 18.38 d | 19.24 d | 20.56 d | 21.48 d | 19.19 C | 80.13 c | 71.11 c | 140.14 c | 130.24 c |
| S. typhi | 14.38 e | 15.28 e | 17.24 e | 18.48 e | 19.88 e | 17.05 C | 15.29 e | 16.97 e | 18.56 e | 19.41 e | 20.69 e | 18.18 CD | 85.36 b | 80.10 b | 150.62 b | 140.78 b |
| p. aeruginosa | 12.79 f | 14.82 f | 16.46 f | 17.55 f | 18.52 f | 16.03 D | 13.41 f | 15.59 f | 17.44 f | 18.22 f | 19.52 f | 16.84 D | 95.61 a | 90.34 a | 170.14 a | 160.14 a |
| Means | 15.77 D | 17.04 C | 19.13 B | 20.12 AB | 21.64 A | 16.71 D | 18.31 C | 20.02 B | 21.25 AB | 22.50 A | 74.41 A | 68.56 B | 131.86 A | 122.85 B | ||
| SEM | 0.23 | 0.56 | 0.37 | 0.67 | 0.50 | 0.36 | 0.44 | 0.40 | 0.70 | 0.49 | 0.56 | 0.60 | 0.88 | 0.99 | ||
| p-value | ||||||||||||||||
| Bacteria (B) | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | ||||||||||
| Concentration (C) | <0.001 | <0.001 | ||||||||||||||
| B × C | <0.001 | <0.001 | ||||||||||||||
| Fungi | Means | Means | MIC | MFC | ||||||||||||
| A. alternate | 20.91 c | 21.51 c | 22.92 c | 24.21 c | 25.25 c | 22.96 B | 21.42 c | 22.24 c | 23.54 c | 25.02 c | 26.42 c | 23.73 B | 70.12 c | 65.33 c | 120.23 d | 110.24 d |
| A. flavus | 21.81 b | 22.32 b | 24.23 b | 26.52 b | 28.22 b | 24.62 AB | 22.32 b | 23.05 b | 24.82 b | 27.33 b | 29.42 b | 25.39 AB | 65.13 cd | 60.27 cd | 110.24 e | 105.34 e |
| F. oxysporum | 19.62 d | 20.55 d | 21.94 d | 23.24 d | 24.23 d | 21.92 C | 20.13 d | 21.22 d | 22.55 d | 24.05 d | 25.43 d | 22.68 BC | 75.52 bc | 70.31 bc | 140.29 c | 130.24 c |
| A. niger | 22.43 a | 24.84 a | 25.79 a | 27.65 a | 29.14 a | 25.97 A | 22.95 a | 25.52 a | 26.38 a | 28.46 a | 30.24 a | 26.71 A | 55.15 d | 50.51 d | 100.23 f | 90.34 f |
| p. solitum | 18.52 e | 19.57 e | 20.88 e | 22.67 e | 23.42 e | 21.01 C | 19.06 e | 20.21 e | 21.42 e | 23.47 e | 24.68 e | 21.77 C | 80.33 b | 75.36 b | 150.14 b | 140.22 b |
| p. crustosum | 16.43 f | 18.22 f | 19.54 f | 21.48 f | 22.61 f | 19.66 D | 17.92 f | 18.93 f | 20.11 f | 22.22 f | 23.87 f | 20.61 D | 95.56 a | 90.25 a | 170.34 a | 155.31 a |
| Means | 19.95 D | 21.17 C | 22.55 B | 24.30 AB | 25.48 A | 20.63 D | 21.86 C | 23.14 B | 25.09 AB | 26.68 A | 73.64 A | 68.67 B | 131.91 A | 121.95 B | ||
| SEM | 0.25 | 0.60 | 0.33 | 0.74 | 0.43 | 0.32 | 0.41 | 0.43 | 0.77 | 0.40 | 0.51 | 0.62 | 0.90 | 0.83 | ||
| p-value | ||||||||||||||||
| Fungi (F) | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | ||||||||||
| Concentration (C) | <0.001 | <0.001 | ||||||||||||||
| F × C | <0.001 | <0.001 | ||||||||||||||
a–f and A–D Mean in the same column within bacterial or fungal isolates within the same nanoparticle concentration with different lowercase letters are significantly different at p < 0.05; differences between means of the same bacterial or fungal isolates within the row and same concentration of different nanoparticles considered significant at p < 0.05; MIC: minimum inhibitory concentration; MBC: minimum bactericidal concentration; MFC: minimum fungicidal concentration. SEM: standard errors means.