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. 2021 Jul 7;11(7):2029. doi: 10.3390/ani11072029

Table 2.

Primer sequences and hybridization temperature.

Gene	Primer Sequences	Hybridization Temperature	Source
β-actin	Forward 5′ATGTGGATCAGCAAGCAGG-3′	57.7 °C	AJ537421 Genne runer
β-actin	Reverse 5′AGAAATGTGTGGTGTGGTCG-3′	57.7 °C	AJ537421 Genne runer
IL-1β	Forward 5′-ATTACCCACCACCCACTGAC-3′	57.7 °C	AJ269472 Genne runer
IL-1β	Reverse 5′-TCTCTTCCACTATGCTCTCCAG-3′	57.7 °C	AJ269472 Genne runer
IL-6	Forward 5′-ACTTCCAAAACATGCCCTGA-3′	59.3 °C	AM490062 (Sepulcre et al., 2007) [22]
IL-6	Reverse 5′- CCGCTGGTCAGTCTAAGGAG-3′	59.3 °C	AM490062 (Sepulcre et al., 2007) [22]
IL-10	Forward 5′-ACCCCGTTCGCTTGCCA-3′	59.3 °C	AM268529 (Picchietti et al., 2009) [23]
IL-10	Reverse 5′-CATCTGGTGACATCACTC-3′	59.3 °C	AM268529 (Picchietti et al., 2009) [23]
Casp-3	Forward 5’-ACGAAGCAGGTCAATCATCC-3’	59.3 °C	DQ345774 Genne runer
Casp-3	Reverse 5’-GCAGTTTAAGGGTATCCAGAGC-3’	59.3 °C	DQ345774 Genne runer
TNF-α	Forward 5′-GCCAAGCAAACAGCAGGAC-3′	60 °C	DQ200910 Genne runer
TNF-α	Reverse 5′-ACAGCGGATATGGACGGTG-3′	60 °C	DQ200910 Genne runer
COX-2	Forward 5′-AGCACTTCACCCACCAGTTC-3′	59.3 °C	AJ630649 (Sepulcre et al., 2007) [22]
COX-2	Reverse 5′-AAGCTTGCCATCCTTGAAGA-3′	59.3 °C	AJ630649 (Sepulcre et al., 2007) [22]
Mx	Forward 5′-GGTCAAGGAGCAGATCAAACAG-3′	57.7 °C	AM228974 Genne runer
Mx	Reverse 5′-CTCGCATCAGGTTAGGGAATC-3′	57.7 °C	AM228974 Genne runer