Table 2.
Summary of chromatographic and mass spectrometric conditions
| Parameter | Optimized Condition |
|---|---|
| Column name | Ascentis Express C18 column (75 mm×4.6 mm×2.7 µm) |
| Mobile phase | Acetonitrile: 5.0 mM ammonium formate (90:10 v/v) |
| Oven temperature | 40 |
| Injected volume (µl) | 10 |
| Flow rate (mL/min) | 0.5 |
| Detector | MS/MS |
| Gas-1 (nebulizer) | 50 |
| Gas-2 (heater gas) | 50 |
| Curtain gas | 18 |
| Collision gas | 6 |
| Ion source | Turbo-ion spray (positive ion mode) |
| Temperature | 450 |
| IS voltage | 4500 V |
| DE clustering potential | 18 |
| Entrance potential | 10 |
| Collision energy | 10 |
| collision cells exit potential | 9 |
| Dwell time (ms) | 200 |
| M/z ion fluoxetine | 310.0/44.1 |
| M/z fluoxetine-d5 | 315.0/44.0 |
| Sample cooler temperature | 10±2 |
| Rinsing solution | Methanol: Water (50:50 v/v) |
| Retention time (fluoxetine), min | 1.5-1.8 |
| Right (fluoxetine d5), min | 1.5-1.8 |
| Run time (min) | 3.5 |
IS: Internal standard, MS: Mass Spectrometry