Table 2.
Primer sequences and target genes used for real-time qPCR reactions.
| Gene | Primer Sequence (5′-3′) | Accession No. |
|---|---|---|
| MSTN | F: ATGCAGATCGCGGTTGATC R: GCGTTCTCTGTGGGCTGACT |
NM_001001461.1 |
| MyoD | F: CAGCAGCTACTACACGGAATCA R: GGAAATCCTCTCCACAATGCTT |
NM_204214.2 |
| Myogenin | F: GGAGAAGCGGAGGCTGAAG R: GCAGAGTGCTGCGTTTCAGA |
NM_204184.1 |
| mTOR | F: CATGTCAGGCACTGTGTCTATTCTC R: CTTTCGCCCTTGTTTCTTCACT |
XM_417614.5 |
| IGF-1 | F: GCTGCCGGCCCAGAA R: ACGAACTGAAGAGCATCAACCA |
NM_001004384.2 |
| Pax3 | F: ACTACCCTGACATTTATACTCG TGCCTGCTTCCTCCATCTAG |
NM_204269.1 |
| Pax7 | F: AGGCTGACTTCTCCATCTCTCCT R: TGTAACTGGTGGTGCTGTAGGTG |
XM_015296832.1 |
| House keeping | ||
| GAPDH | F: CAACCCCCAATGTCTCTGTT R: TCAGCAGCAGCCTTCACTAC |
NM205518 |
| U6 | TTCAGGCTCTTGGACGATTT CCGCTATTCCCAAGACTGAA |
NM_001277862.1 |
MSTN: myostatin; MyoD: myogenic determination factor; MyoG: myogenin; mTOR: mechanistic target of rapamycin; IGF-1: insulin-like growth factor 1; Pax: paired-box.; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.