Figure 7.

Schematic representation of circRNAs validation using divergent primer and sanger sequencing confirming head-to-tail splicing in circRNAs products. The black vertical line represents the back-spliced junction sites. Green and red horizontal lines indicate the 5′ and 3′ ends of the circRNA sequence, respectively. Note: (A–I) Divergent primers used in the amplification of circular junctions. Arrowheads in the left and right direction represent divergent primers. The downward red arrow represents the back spliced junction. The black squares represent exons. The curves in different colors represent the results of Sanger sequencing.