Table 2.
Primers used for quantitative real-time polymerase chain reaction (qRT-PCR).
| Gene | Sequence (5′-3′) | Product Length (bp) | Annealing Temperature (℃) |
Accession Number |
|---|---|---|---|---|
| FSHR | F: ACCACACGTGCCTCTGTGAA R: GCTCCCTTCGGAATGACTCT |
136 | 59.17 | NM_205079.1 |
| LHR | F: CGGATACACAACGATGCCCT R: TTTATCCAGAGGCGGCAGTC |
159 | 59.82 | NM_204936.1 |
| VLDLR/LR8 | F: AACGAGGCAGTCTATGGTGC R: TGTTGAATCCTCCACATCTCAG |
271 | 57.79 | NM_205229.1 |
| VTGII | F: AACTACTCGATGCCCGCAAA R: ACCAGCAGTTTCACCTGTCC |
179 | 58 | NM_001031276.1 |
| ApoVLDL-II | F: CCTTAGCACCACTGTCCCTG R: AGCTCTAGGGGACACCTTGT |
130 | 58 | NM_205483.2 |
| ApoB | F: ACACTTCGGGCTATTGGA R: TGCCTGTATGGCTGCTTT |
129 | 60 | NM_001044633.1 |
| LDLR | F: GGGAACCTCTATTGGGCCG R: CAACATGGGATCCAACGCGA |
270 | 59.85 | NM_204452.1 |
| ER-α | F: TGTGCTGTGTGCAACGACTA R: CAGGCCTGGCAACTCTTTCT |
167 | 57 | NM_205183.2 |
| ER-β | F: GGCTGCAACCCGTGTAAAAG R: GCCCAGCCAATCATGTGAAC |
189 | 58 | NM_204794.2 |
| PPAR-α | F: AGGCCAAGTTGAAAGCAGAA R: TTTCCCTGCAAGGATGACTC |
155 | 60 | NM_001001464.1 |
| PPAR-γ | F: TGACAGGAAAGACGACAGACA R: CTCCACAGAGCGAAACTGAC |
164 | 59 | NM_001001460.1 |
| MTTP | F: GTTCTGAAGGACATGCGTGC R: GATGTCTAGGCCGTACGTGG |
120 | 58 | NM_001109784.2 |
| FAS | F: GCTAAGATGGCATTGCACGG R: TCCATTCAGTTCCAGACGGC |
135 | 58 | NM_205155.1 |
| Bcl- 2 | F: ATCGTCGCCTTCTTCGAGTT R: ATCCCATCCTCCGTTGTCCT |
150 | 59 | Z11961.1 |
| Bax | F: GTGATGGCATGGGACATAGCTC R: TGGCGTAGACCTTGCGGATAA |
90 | 58 | XM_422067.4 |
| Caspase-3 | F: ACTCTGGAATTCTGCCTGATGACA R: CATCTGCATCCGTGCCTGA |
129 | 59 | NM_204725.1 |
| p53 | F:GAGATGCTGAAGGAGATCAATGAG R: GTGGTCAGTCCGAGCCTTTT |
145 | 58 | X13057.1 |
| IL-10 | F: GGAGAGAGCGGAGGTTTCG R: TCCCGTTCTCATCCATCTGC |
118 | 59.86 | XM_025143715.1 |
| IL-6 |
F: CTGCAGGACGAGATGTGCAA R: AGGTCTGAAAGGCGAACAGG |
175 | 60.67 | NM_204628.1 |
| IL-1β | F: TGCCTGCAGAAGAAGCCTCG R: GACGGGCTCAAAAACCTCCT |
204 | 60.25 | NM_204524.1 |
| GAPDH | F: TCCTCCACCTTTGATGCG R: GTGCCTGGCTCACTCCTT |
144 | 60 | NM_204305.1 |