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. Author manuscript; available in PMC: 2022 Jan 27.
Published in final edited form as: J Am Chem Soc. 2021 Jan 12;143(3):1341–1347. doi: 10.1021/jacs.0c05605

Figure 6.

Figure 6.

Validation of the fluoroacetyl labeling sites on known protein substrates by proteomics analysis. (A) Scheme for cellular pro-metabolite incorporation, lysis, and the histone proteins extraction; or immunoprecipitation of α-tubulin after cellular pro-metabolite incorporation. (B) Summary of the F–Ac labeling sites (red) on representative histone proteins H2B, H4 (PDB: 1kx5), and α-tubulin (PDB: 1tub). Details of the proteomics results are available in the Supporting Information (Figures S20–S22).