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. 2021 Jun 30;10(7):1060. doi: 10.3390/antiox10071060

Figure 5.

Figure 5

Figure 5

Prdx1 (WT, C52A, and C173A) and Prdx2 WT interact with ASK1 independently of AnxA2. The fluorescent signal (red dots) emitted by the mLumin protein upon proteins complementation in AnxA2 KO HEK293 MSR cells (i.e., the interaction of the ASK1-LN with Prdx1-LC) is shown. BiFC signal upon (A) Prdx1 WT:ASK1, (B) Prdx1 C52A:ASK1, (C) Prdx1 C173A:ASK1, (D) Prdx2 WT:ASK1, (E) Prdx1 WT:STAT3, and (F) Prdx2 WT:STAT3 interaction. The treatments from left to right were: no treatment, 100 µM H2O2 for 30 min, X/XO (8 µM X and 1 mU/mL XO) for 18 h, and 0.8 µM auranofin for 18 h. (G) Quantified mLumin/GFP values in AnxA2 KO HEK293 MSR cells; Prdx1 WT:STAT3 and Prdx2 WT:STAT3 were the controls. All images were captured with the total magnification of 100×. The GFP images used for normalization are presented in Figure S7. The results are representative of n = 3 independent experiments. All bar charts in this figure represent the mean ± SD. * p ≤ 0.05; *** p ≤ 0.001; ns not significant; based on an unpaired ANOVA test.