Table 2.
Point mutations in selected ESBL enzymes.
| Amino Acid Change | Number of Mutations (Amino Acid Position) | ||||||
|---|---|---|---|---|---|---|---|
| from | to | CTX-M * | OXY * | PER * | SHV * | TEM * | VEB * |
| A | C | 7 (22) | 2 (7) | ||||
| A | D | 36 (32/131/157) | 3 (114) | 1 (264) | 1 (230) | ||
| A | E | 28 (35/46/157/222/236) | 21 (230) | 2 (114/166) | 1 (38) | ||
| A | G | 158 (16/64/73/131/136/157/297) | 10 (240) | 1 (7) | 3 (205/259) | 2 (235/280) | |
| A | I | 1 (16) | |||||
| A | K | 22 (64/244) | |||||
| A | M | 1 (73) | |||||
| A | N | 36 (35) | |||||
| A | P | 78 (59/78/272) | 1 (15) | 1 (183) | |||
| A | Q | 1 (157) | 2 (151) | ||||
| A | R | 1 (222) | 1 (151) | 1 (185) | |||
| A | S | 102 (35/46/151/235/244) | 2 (240) | 4 (109) | 1 (23) | 3 (9/40/266) | |
| A | T | 110 (16/18/35/90/120/131/137/161/222/244) | 108 (16/30/32/38/56/129/171/208/240/284) | 1 (11) | 14 (20/23/63/90/125/135/157/161/204) | 12 (9/183/235/280) | 4 (248) |
| A | V | 74 (16/35/88/131/135/137/163) | 32 (16/233) | 20 (20/63/125/135/137/145/157/188/205/218/264/288) | 18 (23/40/182/222/266/276) | ||
| A | W | 1 (299) | |||||
| C | N | 1 (9) | |||||
| C | R | 1 (134) | |||||
| C | S | 1 (9) | |||||
| D | A | 69 (36) | 1 (39) | 4 (155) | 3 (195/231) | ||
| D | E | 160 (218/303) | 1 (242) | 2 (168/230) | 2 (155/177) | ||
| D | G | 179 (259/270) | 4 (115/195/230/231) | 2 (113/161) | |||
| D | H | 23 (36/292/303) | 2 (112) | 1 (161) | |||
| D | K | 2 (303) | |||||
| D | N | 40 (36/218/270/303) | 28 (39/58) | 1 (195) | 3 (36/113/174) | ||
| D | P | 1 (292) | 1 (33) | ||||
| D | Q | 1 (218) | |||||
| D | S | 25 (36/65/256/303) | |||||
| E | A | 106 (47/177) | |||||
| E | D | 35 (98/169) | 22 (92/99/149/278) | 2 (29/273) | 1 (26) | ||
| E | G | 1 (47) | 1 (33) | 3 (59/75/177) | 2 (164/237) | ||
| E | K | 3 (47/132/169) | 1 (278) | 37 (29/100/256/289) | 82 (26/62/102/166/237) | ||
| E | N | 1 (169) | |||||
| E | P | 1 (107) | |||||
| E | Q | 85 (98/132) | 4 (92) | 8 (119/193/224) | 1 (75) | ||
| E | R | 1 (256) | 1 (237) | ||||
| E | T | 1 (98) | |||||
| E | V | 3 (23) | 1 (237) | ||||
| F | A | 10 (12) | |||||
| F | L | 1 (12) | 3 (19) | 3 (14/228) | |||
| F | M | 72 (12) | |||||
| F | S | 21 (12) | 1 (162) | ||||
| F | W | 1 (22) | |||||
| F | Y | 2 (64) | |||||
| G | A | 31 (25/217) | 4 (149/190) | 6 (158/191/255) | |||
| G | C | 3 (47/132/169) | 1 (258) | ||||
| G | D | 1 (159) | 11 (98/154/167/245) | 4 (39/90/194/236) | |||
| G | E | 1 (25) | 1 (41) | 2 (158/296) | 1 (216) | ||
| G | H | 1 (304) | |||||
| G | K | 1 (126) | |||||
| G | N | 1 (53) | 1 (236) | ||||
| G | R | 4 (54/234/304) | 1 (296) | 2 (154/236) | |||
| G | S | 27 (25/213/234/253/304) | 30 (24/75) | 42 (65/155/167/255) | 40 (194/236) | ||
| G | V | 2 (194/263) | |||||
| H | D | 2 (134/154) | |||||
| H | F | 1 (123) | |||||
| H | K | 1 (152) | |||||
| H | L | 2 (134) | 4 (285) | ||||
| H | N | 1 (156) | |||||
| H | Q | 74 (152) | 1 (22) | ||||
| H | R | 5 (24/151) | |||||
| H | T | 1 (107) | |||||
| H | Y | 3 (123) | |||||
| I | A | 1 (69) | 2 (5/97) | ||||
| I | F | 1 (119) | 11 (6) | 1 (11) | |||
| I | L | 1 (150) | 5 (33) | 3 (61/194) | 2 (297/302) | ||
| I | M | 4 (111) | 1 (275) | 1 (259) | |||
| I | P | 4 (3) | |||||
| I | R | 1 (11) | |||||
| I | S | 1 (300) | |||||
| I | T | 13 (69) | 7 (5/40/97) | 2 (275/297) | 1 (275) | 1 (18) | |
| I | V | 170 (69/108/266/275) | 7 (111) | 15 (61/162/194/245/258) | 3 (6/58) | 5 (125/171/256) | 14 (18) |
| K | A | 5 (109/110/299) | 23 (102) | 3 (102) | |||
| K | D | 1 (214) | |||||
| K | E | 36 (93/122/269) | 27 (180) | 1 (113) | 1 (32) | ||
| K | G | 1 (214) | |||||
| K | H | 73 (4/214) | |||||
| K | L | 1 (148) | 20 (140) | ||||
| K | N | 80 (3/214/286) | 20 (101) | 2 (209/271) | |||
| K | P | 81 (110) | |||||
| K | Q | 217 (4/44/94/109/269/286) | 2 (196) | 1 (144) | |||
| K | R | 103 (3/44/94/109/122/229/251/299) | 7 (196/246) | 5 (251/271) | 2 (237) | ||
| K | S | 7 (110/214) | |||||
| K | T | 91 (3/269) | 1 (190) | ||||
| L | A | 4 (9/14/23/210) | 2 (256) | ||||
| L | F | 121 (14/24/30/130/305) | 1 (84) | 6 (124/133/189) | 23 (19/55/100/136) | 1 (56) | |
| L | G | 2 (30/70) | |||||
| L | I | 111 (9/149/166/276) | 24 (2) | 1 (256) | 4 (19/135/218) | ||
| L | M | 5 (9/22/102/207/242) | 23 (2/11) | 4 (75/260) | 2 (17/237) | 3 (47/219) | |
| L | N | 1 (305) | |||||
| L | P | 4 (130/149/212) | 1 (262) | 4 (38/62/102/301) | 1 (49) | ||
| L | Q | 2 (60/180) | 73 (33/62) | ||||
| L | R | 5 (8/102/133/185) | 1 (10) | ||||
| L | S | 3 (14/23/305) | |||||
| L | V | 193 (9/60/101/153/216) | 3 (305) | 2 (159/210) | 4 (28/38/100/247) | ||
| L | Y | 15 (224/305) | |||||
| M | A | 69 (14) | |||||
| M | G | 1 (197) | |||||
| M | I | 1 (86) | 35 (12/141) | 1 (160) | 3 (80) | 10 (66/67/153/180) | |
| M | K | 4 (3) | |||||
| M | L | 105 (228) | 20 (12) | 3 (160) | 1 (80) | 17 (67) | |
| M | T | 21 (14) | 34 (180) | ||||
| M | V | 7 (80/140/228) | 11 (67/184) | ||||
| N | D | 89 (62/125/143) | 36 (54/90/200/256) | 3 (308) | 2 (269/291) | 12 (272) | 6 (294) |
| N | E | 1 (86) | |||||
| N | G | 12 (66/100/125) | |||||
| N | H | 25 (36/65/256/303) | 10 (90) | 2 (269) | 4 (134/173) | ||
| N | I | 1 (270) | 1 (173) | ||||
| N | K | 2 (100/209) | 1 (169) | ||||
| N | L | 1 (100) | |||||
| N | Q | 141 (100/209) | |||||
| N | S | 27 (100/103/117/181) | 3 (98/272) | ||||
| N | T | 1 (66) | 1 (168) | ||||
| N | W | 1 (209) | |||||
| N | Y | 1 (115) | 1 (200) | ||||
| P | A | 3 (29/178/194) | 3 (123) | 1 (143) | |||
| P | G | 1 (268) | |||||
| P | H | 1 (178) | |||||
| P | K | 105 (99) | 23 (91) | ||||
| P | L | 3 (178/188) | 1 (269) | 3 (156/268/284) | |||
| P | N | 4 (91) | |||||
| P | Q | 95 (156/178/185/285) | |||||
| P | S | 18 (178/283) | 1 (170) | 7 (18/27/190/236/243/284) | 1 (143) | ||
| P | T | 29 (29/178/283) | 1 (123) | ||||
| Q | A | 77 (11/165/220) | |||||
| Q | D | 1 (199) | |||||
| Q | E | 35 (165/284) | 1 (165) | ||||
| Q | H | 1 (68) | 23 (34/156) | 2 (223/293) | 1 (88) | ||
| Q | I | 1 (68) | |||||
| Q | K | 2 (11/223) | 2 (94/183) | 38 (4/37) | |||
| Q | L | 3 (11/165/199) | 4 (156/270) | 1 (129) | |||
| Q | P | 1 (116) | 2 (175/214) | 1 (203) | |||
| Q | R | 198 (11/68/239) | 27 (35/156) | 1 (158) | 1 (37) | 3 (97/204) | |
| Q | S | 128 (33/51/165) | |||||
| Q | T | 10 (165) | |||||
| Q | V | ||||||
| R | A | 4 (176/271) | |||||
| R | C | 1 (72) | 9 (162/241) | ||||
| R | G | 2 (10/175) | 2 (232) | 2 (118/241) | |||
| R | H | 25 (10/271/291) | 9 (43/68/194) | 5 (54/72/219) | 22 (162/238/241) | ||
| R | K | 131 (10/50/105/208) | |||||
| R | L | 37 (10/72/195) | 7 (76/175/222/232/290) | 4 (241/271) | |||
| R | M | 1 (97) | |||||
| R | N | 1 (43) | |||||
| R | P | 73 (76/105) | 1 (175) | 1 (41) | |||
| R | Q | 74 (10/50/208/221/290) | 4 (257/307) | 6 (202/271) | |||
| R | S | 4 (10/105/175) | 10 (43/225) | 17 (54/109/219) | 37 (63/162/241) | ||
| R | T | 1 (41) | |||||
| R | V | 2 (72) | |||||
| S | A | 110 (52/67/111/296) | 45 (25/89/103) | 1 (280) | |||
| S | C | 1 (245) | 1 (4) | 1 (221) | |||
| S | D | 4 (2) | |||||
| S | E | 2 (280) | |||||
| S | F | 1 (12) | |||||
| S | G | 101 (111/134/141/158/237/245) | 39 (143/231) | 2 (18) | 2 (36/144) | 7 (128/264) | |
| S | H | 2 (289) | |||||
| S | I | 3 (134/254) | 1 (192) | 2 (286) | |||
| S | K | 36 (158/296) | |||||
| S | L | 1 (220) | |||||
| S | N | 6 (26/237/254/289) | 29 (4/29/157/275) | 3 (81/213) | 1 (122) | ||
| S | P | 1 (104) | |||||
| S | Q | 1 (97) | |||||
| S | R | 83 (5/237/289) | |||||
| S | T | 275 (67/97/129/141) | 37 (4/44/174) | 10 (12/37/121/135) | 1 (117) | 3 (128/233) | 2 (131) |
| S | V | 2 (219) | 2 (89) | ||||
| T | A | 192 (13/17/19/21/34/144/206/211/226/281/302) | 11 (13/165/293) | 9 (16/69/82/252) | 9 (25/176/219) | ||
| T | C | 81 (19) | |||||
| T | D | 1 (233) | |||||
| T | G | 3 (17/19) | 1 (178) | ||||
| T | H | 2 (226/233) | |||||
| T | I | 6 (176/182/226/278) | 48 (9/61) | 4 (152/212) | 1 (267) | ||
| T | K | 3 (34/170/211) | 3 (202) | 1 (186) | |||
| T | M | 134 (13/17/226) | 8 (168) | 1 (283) | 19 (261) | 10 (104) | |
| T | N | 1 (176) | 2 (16/212) | 1 (186) | |||
| T | P | 90 (19/21) | 1 (16) | 1 (112) | |||
| T | S | 130 (21/127/170/179/182/192/193/206) | 34 (9/184) | 3 (95/221) | 4 (129/160/283) | ||
| T | V | 108 (144/206) | 1 (61) | 3 (24) | |||
| V | A | 98 (27/85/106/248) | 20 (161) | 1 (290) | 2 (86/91) | 2 (19) | |
| V | E | 1 (78) | 7 (19) | ||||
| V | F | 1 (293) | 3 (9) | 2 (153) | 1 (258) | ||
| V | G | 3 (37/247) | |||||
| V | I | 175 (2/20/37/114/159/293/301) | 2 (261) | 2 (235) | 1 (130) | 6 (82) | |
| V | L | 22 (20/91) | 11 (17/94) | 3 (17) | 1 (86) | ||
| V | M | 35 (2/37) | 5 (86/91/95/277) | ||||
| V | P | 1 (27) | |||||
| V | Q | 2 (27/106) | |||||
| V | R | ||||||
| V | S | 2 (225/247) | 1 (42) | ||||
| V | T | 68 (247) | |||||
| W | C | 1 (163) | |||||
| W | G | 2 (163) | |||||
| W | L | 1 (163) | |||||
| W | P | 1 (176 | |||||
| W | R | 1 (246) | 1 (71) | 5 (163) | |||
| Y | C | 10 (31) | |||||
| Y | F | 1 (31) | 8 (5) | 1 (103) | |||
| Y | G | ||||||
| Y | H | 22 (31) | 1 (144) | 4 (115) | 2 (5/108) | ||
| Y | N | 1 (103) | |||||
| Y | S | 1 (31) | 1 (103) | ||||
| Y | W | 1 (71) | |||||
Total numbers of mutations: CTX-M: 6344, OXY: 904, PER: 162, SHV: 418, TEM: 534, VEB: 61. Numbers of amino acid insertions: CTX-M: 6, OXY: 10, PER: 0, SHV: 9, TEM: 0, VEB: 0. Numbers of amino acid deletions: CTX-M: 3, OXY: 8, PER: 0, SHV: 2, TEM: 3, VEB: 1. * Total numbers of analyzed sequences: CTX-M (216), OXY (48), PER (15), SHV (199), TEM (199), VEB (27).