Table 3.

Additional highly conserved residues.

>90%	80 ≤ 90%	70 ≤ 80%	50 ≤ 80%
G51(NS), Y77(FST), G86(NS), L118(MP), P119(G), G179(AS), R185(N), N211(RS) P223(G), F246(LVY), D264(HNQS), A266(G), V340(GIT), T343(ASV), P358(CL), R363(KN), P368(ATN), G387(DN), E602(Q), Q774(IMRS), R780(DL), A890(GISTV), E893(GN), F948(VY), G994(DS)	I6(TV), F94(AILM), S144(ADFN), V172(AIMT), A279(GST), T394(NS), A401(CSV), V416(ACIM), A457(GSV), T473(ASV), N820(ADLMQS), A889(CSV), V905(AIL), G911(AS), N941(HST), V946(ACFIT), G996(AS)	N109(ADS), V122(AIST), D156(EHNS), R168(AG KQST), Q210(EHNSY), A299(ELPST), L350(ACIV), Q360(AGHR), F396(ALV), M435(ACLSTV), V448(AIT), F453(LY), L492(IMQ), Q469(ES), Q928(DEKLMV), T933(ALMV), A942(G), F982(LMT)	A16(NST), M355(ITV), T365(AIMSV), V372(AI), T431(ASV), S434(AGST), I445(MSTY), I446(FLM(V)), S471(AT(CG)), S375(ACSV(T)), M478(IMTV(A)), T489(S(K)), V884(AI), L891(Q(M)), D924(N(S)), R1000(Q(KL))

Residues were chosen from the relatively highest conserved category after analysis by ConSurf [67,68], and conservation ranges between 50% and 100% (Table S1), excluding the residues mentioned already in Table 2. Residues in brackets (AA) indicate alternative residues among the 135 pumps. Double brackets at “50 ≤ 80%” ((AA)) indicate < 1% occurrence. Numbering and amino acid labeling as in AcrB-Ec. The conservation heat map based on these residues can be seen in Figure 3a and Figure S3 (dark purple). Underlined, mentioned explicitly in this article.