Table 2.

Summary of developed isothermal techniques.

Technique	Template	Temperature 1	Enzyme	Reference
Helicase-dependent amplification (HDA)	DNA	65 °C	Helicase	[147]
Insulated isothermal PCR (iiPCR)	DNA	95 °C	Taq DNA polymerase	[24]
Insulated isothermal reverse-transcription PCR (iiRT-PCR)	RNA	95 °C	Taq DNA polymerase M-MLV reverse transcription	[148]
Loop-mediated isothermal amplification (LAMP)	DNA	65 °C	Bst DNA polymerase	[23]
Reverse transcription loop-mediated isothermal amplification (RT-LAMP)	RNA	65 °C	Bst DNA polymerase AMV reverse transcription	[149]
Multiple displacement amplification (MDA)	DNA	30 °C	Φ29 DNA polymerase	[150]
Nucleic acid sequence-based amplification (NASBA)	RNA	50 °C	T7 RNA polymerase RNase H AMV reverse transcription	[151]
Rolling circular amplification (RCA)	DNA	30 °C	Phi29 Bst DNA polymerase Vent exo-DNA polymerase T7 RNA polymerase	[152]
Recombinase polymerase amplification (RPA)	DNA RNA	37 °C	DNA polymerase	[153]
Strand displacement amplification (SDA)	DNA	60 °C	DNA polymerase	[154]

¹ Average temperature used in respective assays.