Table 5.

Comparison of conventional PCR to iiPCR and LAMP assays and procedures.

Properties	PCR	iiPCR	LAMP
Temperature	Cycles through 3 temperatures 55–95 °C	Constant temperature drives temperature gradient 15–30 °C	Constant temperature 60–65 °C
Equipment	Thermocycler	Specialized reaction tube Fluorescence-based detector	Heat source
Field-deployable	No	Yes	Yes
Reaction time	At least 90 min	≤60 min	<30 min
Sensitivity	Starts at nanograms	Starts at nanograms	Starts at femtograms
Specificity	Requires specific primer design Prone to errors	Requires specific primer design Prone to errors	Tolerates combination of primer designs
Visualization	Only through gel electrophoresis	Real-time available	Real-time available
Template prep	Requires purification	Requires purification	Tolerates impurities
Cost	$$$	$$	$