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. 2021 Jul 7;10(7):828. doi: 10.3390/antibiotics10070828

Figure 1.

Figure 1

Short-circuit current (Isc) recording experiments on brushed nasal epithelial cells. Case 1 to 3 are short-circuit currents from CF patients with F508del/Unknown genotype treated with KaftrioTM. Cells were treated with (green line) or without (dark red line) a combination of VX-445 (3 μM), VX-661 (3 μM) and VX-770 (100 nM) for 24 h. The black arrows indicate: A = addition of Amiloride (100 μM) for ENaC channel activity inhibition, F = addition of Forskolin (10 μM), IBMX (3-Isobutyl-1-methylxanthine) (100 μM) for activation of the transepithelial cAMP-dependent current (including CFTR channel activity) and I = addition of Inh-172 (5 μM) for CFTR specific inhibition. All chemicals were added to the apical/mucosal side of cells.