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. 2021 Jul 8;12:682489. doi: 10.3389/fphys.2021.682489

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Copyright © 2021 Rigosi and O’Carroll.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Electrophysiological set up for long-lasting recordings in perfused insects. We modified our existing set up to perform electrophysiological recordings while constantly perfusing the insect head capsule. (A) A low-noise peristaltic perfusion pump was fed into a micro ML-6 manifold apparatus that received also 5 PE-50 tubing from a 6-valve computer-controlled gravity system, each of these were connected to a 20 ml syringe filled with treatment solutions. (B) Close up of the insect head showing the inlet tubing, the outlet and the recording electrode (the example shown is in the bumblebee Bombus terrestris rather than Eristalis). (C) Schematic drawing of the perfusion system.