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. 2021 Jun 23;10(7):1006. doi: 10.3390/antiox10071006

Figure 6.

Figure 6

Oxidative stress in heart. (a) Lysates of heart from WKY C, SHR C, SHR + (+/−) alpha lipoic acid (ALA) 250 µmol/kg/day, SHR + (+/−) ALA 125 µmol/kg/day, SHR + (+) ALA 125 µmol/kg/day, SHR + (−) ALA 125 µmol/kg/day were immunoblotted using the Oxyblot kit; (b) The bar graph reports the level of oxidized proteins measured in optical density unit (ODU); (c) Sections of heart processed for the 8-oxo-dG immunohistochemistry (IHC). Arrow: positive nucleus. Calibration bar 25 µm; (d) The bar graph shows the quantification of the number of positive cells per high-power field (HPF) (40×). Data are mean ± S.E.M. * p < 0.05 vs. WKY C; # p < 0.05 vs. SHR C.