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. 2021 Jun 23;10(7):1006. doi: 10.3390/antiox10071006

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Apoptosis in heart. (a) Lysates of heart from WKY C, SHR C, SHR + (+/−) alpha lipoic acid (ALA) 250 µmol/kg/day, SHR + (+/−) ALA 125 µmol/kg/day, SHR + (+) ALA 125 µmol/kg/day, SHR + (−) ALA 125 µmol/kg/day were immunoblotted using specific anti caspase-3; (b) The bar graph indicates the ratio of densitometric analysis of bands and GAPDH levels used as loading control, considering the WKY C group as reference. Blots are representative of one of three separate experiments; (c) Sections of heart from WKY C and SHR C processed for TUNEL staining. Arrow: apoptotic nucleus. Calibration bar 50 µm; (d) The bar graph shows the quantification of the number of positive cells per high-power field (HPF) (20×). Data are mean ± S.E.M.