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. 2021 Jul 8;10(7):833. doi: 10.3390/antibiotics10070833

Figure 2.

Figure 2

Effect of human serum albumin (HSA) and pooled normal human serum (HS) on the expression of antibiotic resistance genes of A. baumannii strains. qRT-PCR of AMA16: (A) AB0057 (B) and ABUH702 (C) strains genes associated with antibiotic resistance expressed in LB, LB supplemented with 3.5% HSA, or in HS. Fold changes were calculated using double ΔΔCt analysis. At least three independent samples were used. LB was used as the reference condition. Statistical significance (p < 0.05) was determined by ANOVA followed by Tukey’s multiple-comparison test, one asterisks: * p < 0.05; two asterisks: ** p < 0.01; and three asterisks: *** p < 0.001.