Figure 4.

Effect of human serum albumin (HSA) and pooled normal human serum (HS) on the expression of biofilm formation genes in A. baumannii strains. qRT-PCR of A118 (A) and AB5075 (B) strains of the csuB, csuA/B, csuE, and ompA genes biofilm formation related in cells cultured in LB, LB supplemented with 3.5 % HSA, or in HS. Fold changes were calculated using double ΔΔCt analysis. At least three independent samples were used. LB was used as the reference condition. Statistical significance (p < 0.05) was determined by ANOVA followed by Tukey’s multiple-comparison test, one asterisks: * p < 0.05; two asterisks: ** p < 0.01 and three asterisks: *** p < 0.001.