Figure 6.

Effect of human serum albumin (HSA) and pooled normal human serum (HS) on the expression of quorum sensing genes in A. baumannii. qRT-PCR of A118 (A) and AB5075 (B) strains of the aidA, abaI, and abaM genes associated with quorum sensing and quorum quenching expressed in LB, LB supplemented with 3.5% HSA, or in HS. Fold changes were calculated using double ΔΔCt analysis. At least three independent samples were used. LB was employed as the reference condition. Statistical significance (p < 0.05) was determined by ANOVA followed by Tukey’s multiple-comparison test, two asterisks: ** p < 0.01 and three asterisks: *** p < 0.001.