Figure 7.

Effect of human serum albumin (HSA) and pooled normal human serum (HS) on quorum sensing in A. baumannii. (A) N-Acyl Homoserine Lactone (AHL) detection in A. baumannii A118 and AB5075 supernatants of bacterial cultured in LB, LB supplemented with 3.5% HSA, or HS, using Agrobacterium tumefaciens as reported strain. The presence of AHL was determined by the development of the blue color. (B) Quantification of 5,5′-dibromo-4,4′-dichloro-indigo was estimated as the percentage relative to C10-AHL standard, measured with ImageJ (NIH). As negative control, HS was used. The mean ± SD is informed. Statistical significance (p < 0.05) was determined by ANOVA followed by Tukey’s multiple-comparison test. one asterisks: * p < 0.05; two asterisks: ** p < 0.01 and three asterisks: *** p < 0.001.