Figure 4.

Anti-AcrA Western blot analysis of samples prepared from BW25113 containing plasmids expressing the indicated AcrA mutant (diluted 4-folds). Sample prepared from plasmid-free BW25113 was also prepared and loaded without dilution (\) to serve as a control to highlight the difference in expression levels. (a) AcrA mutants are expected to affect AcrA–AcrA interaction during pump assembly. (b) AcrA mutants forming intra-subunit disulfide bond to be trapped in an inactive conformation. Addition of BME did not lead to an observable change in mobility.