Table 1.

MIC values (µg/mL) of BW25113 or BW25113∆acrB strains containing the indicated plasmid encoding AcrB mutants defective in proton translocation pathway.

Substrate 1	NOV	ERY	TPP	EtBr	R6G	NA
BW25113∆acrB containing
/	4	4	4	8	8	1
WT	128	64	256	128	256	4
D407A	4	4	4	8	8	ND
D408A	4	4	4	8	8	ND
K940A	8	4	4	8	8	ND
R971A	8	4	4	8	16	ND
T978A	8	8	16	32	128	ND
BW25113 containing
/	256	64	1024	512	1024	4
WT	512	128	1024	512	1024	4
D407A	128	64	1024	256	512	4
D408A	128	64	1024	512	1024	4
K940A	256	64	1024	512	1024	4
R971A	128	32	1024	512	1024	4
T978A	256	64	1024	512	1024	2

¹ NOV, novobiocin. ERY erythromycin, TPP tetraphenylphosphonium, EtBr ethidium bromide. R6G, rhodamine 6G, NA, nalidixic acid. ND, not determined.