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. 2021 Jun 28;10(7):1037. doi: 10.3390/antiox10071037

Table 2.

HPLC analysis.

Protocol Column Detection Derivatization Reagent Sample Matrix Limits of Detection Ref.
RP-HPLC Hypersil ODS Fluorescent 3-mercaptopropionic acid/o-phthaldehyde muscle 0.005 mmol/kg [32,33]
RP-HPLC APEX ODS Fluorescent 3-mercaptopropionic acid/o-phthaldehyde muscle 0.005 mmol/kg [34]
RP HPLC Hypersil ODS Fluorescent 3-mercaptopropionic acid/o-phthaldehyde muscle 0.005 mmol/kg [35]
JLC-300 LCR-6 n.a n.a. muscle 0.005 mmol/kg [36]
RP HPLC XBridge BEH Fluorescent AccQTag muscle n.a. [38]
RP HPLC Hypersilica UV (210 nm) n.a. muscle 3 and 10 μM [41]
RP HPLC HILIC silica UV (214 nm) n.a muscle [39]
RP HPLC Hypersil ODS Fluorescent 3-mercaptopropionic acid/o-phthaldehyde human plasma [18]
UPLC Acquity Peptide BEH C18 MS n.a. human plasma [40]
RP HPLC Jupiter C18 Fluorescent carbazole-9-carbonyl chloride (CFC) human serum, animal feed 15 nM [28]