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. Author manuscript; available in PMC: 2021 Sep 25.
Published in final edited form as: Nat Immunol. 2021 Mar 25;22(4):485–496. doi: 10.1038/s41590-021-00896-3

Fig. 2 |. Tumor-expressed DAPK3 shapes immune surveillance.

Fig. 2 |

a, b, Tumor volume of shRNA-transduced (a) MCA205 and (b) B16F10 subcutaneously transplanted into C57BL/6J wild type (WT) mice (n=8 per group). c, Flow cytometry of tumor-infiltrating leukocytes in MCA205 tumor suspensions isolated from WT mice on Day 6 (n=7 for shControl, n=8 for shDapk3#1 and shSting1#1). d, Tumor volume of shRNA-transduced MCA205 subcutaneously transplanted into WT and Ifnar1-KO mice (n=8 per group). Tumor size on Day 18 is represented (right panel). e, Tumor volume of shDapk3#1-transduced MCA205 rescued with lentiviral DAPK3 (WT) or DAPK3 (D161A) prior to sub-cutaneous transplantation into WT mice and Ifnar1-KO mice (n=8 per group). f, Flow cytometry of intracellular pTBK1 and pIRF3 in MCA205-GFP tumor cells (CD45−GFP+) and tumor-infiltrating DCs (CD45+CD11b−CD11c+) in MCA205-GFP tumor suspensions isolated from WT mice on Day 6 (n=6 per group). Values represent percentage of each total cell population. Data in (a-f) are representative of three independent experiments. Values represent mean ± s.d. *P<0.05, **P<0.01, and ***P<0.001. Statistical comparisons were conducted using two-tailed t-test (a-f).