Figure 3.

Cytotoxicity of insulin species glycated in the presence of HT. SHSY5Y cells were exposed for 24 h to insulin samples glycated with d-ribose in the presence of different concentrations of HT (0, 0.5, 1, 2 mM) and the cell viability was evaluated by the MTT assay. Data are expressed as average percentage of MTT reduction ±SD relative to control cells from triplicate wells from 5 separate experiments (p < 0.01). Other experimental details are described in the Methods section. *** p ˂ 0.001 versus CTR.