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Glycated insulin-HT interaction monitored by intrinsic fluorescence spectroscopy. Tyrosine fluorescence emission has been detected in native insulin (Ins) and insulin glycated by d-ribose (InsRib) in the absence and in the presence of HT at different molar ratio (1:0, 1:0.5, 1:1, 1:2). Insulin working concentration was 10 µM. Other experimental details are described in the Methods section.
