Figure 6.

Effect of HT on insulin AGE toxicity. SHSY5Y cells were coincubated with glycated insulin and HT (1:0, 1:1, 1:2 molar ratio) and the cell viability was evaluated after 24 and 48 h by the MTT assay. CTR: cells treated with non-glycated insulin; HT: cells treated with HT at the higher working concentration; data are expressed as average percentage of MTT reduction ±SD relative to control cells from triplicate wells from 5 separate experiments. Other experimental details are described in the Methods section. *** p ˂ 0.001 versus CTR.