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. 2021 Jul 15;10(7):1127. doi: 10.3390/antiox10071127

Figure 7.

Figure 7

Role of HT in AGE-induced oxidative stress. SHSY5Y cells were co-incubated with glycated insulin (InsRib) and HT (1:0, 1:1, 1:2 molar ratio) and the effect of HT was evaluated in ROS production by DCFH-DA assay (A), SOD2 expression (B) and IRT1 expression (C) by western-blot analysis. CTR: cells treated with non-glycated insulin; HT: cells treated with the higher working concentration of HT; CTR+: cells treated with 1.0 mM H2O2. Data are expressed as average ± S.D from five independent experiments carried out in triplicate. Other experimental details are described in the Methods section. *** p ˂ 0.001 versus CTR, ### p ˂ 0.001 versus InsRib.