Figure 3.

An intact microtubule-cytoskeleton is necessary for the maintenance of inner-cellular Tom20 protein abundance gradients. Cells were mock-treated (A) or incubated for 48 h with the microtubule depolymerizing compound, nocodazole (B). Cells were labeled with antisera against α-tubulin and Tom20. (C) Normalized slopes of the fluorescence intensity gradients of control and nocodazole treated cells decorated with antiserum against Tom20. For the analysis, overlapping mitochondria were excluded. Each grey rhomb represents one cell. Black dot: mean. Error bars: Standard error of the mean. *** p = 0.001 (paired t test analysis). (D) STED image of a cell treated for 48 h with nocodazole and labeled with antiserum against Tom20. Overview image (top) and magnifications of perinuclear mitochondria and peripheral mitochondria from the indicated areas. (E) Analysis of the Tom20 cluster density in (D). Each sphere represents one mitochondrion within the image. The size of the spheres corresponds to the area of the mitochondrion. Scale bars: 0.5 µm (D: magnifications), 10 µm (D: overview), and 40 µm (A,B).