Figure 2.

βHB potentiates the antioxidant defense in a Trx1-dependent manner. (A) Trx1 knockdown inhibits βHB-induced Trx1 upregulation. Cardiomyocytes were treated with Trx1 short hairpin RNA (shTrx1) adenovirus vector for 3 days and with 1 mM βHB for 16 h. The expression of Trx1 was examined using Western blot analysis. n = 5–6. (B) βHB inhibits H₂O₂-induced cardiomyocyte death in a Trx1-dependent manner. (C) A high dose of βHB (10 mM) did not significantly inhibit H₂O₂-induced cardiomyocyte death. (B,C) Cardiomyocytes were treated with 1 or 10 mM βHB for 16 h and were then treated with 100 µM H₂O₂ for 6 h. The cardiomyocyte viability was examined using trypan blue dye exclusion. n = 4. * p < 0.05 (A–C).