Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 20;10(7):1153. doi: 10.3390/antiox10071153

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

βHB potentiates AMPK function in a Trx1-dependent manner. (A) AMPKα exhibits intermolecular disulfide bond formation upon oxidation. Cardiomyocytes were treated with 100 µM H₂O₂ for 30 min. Western blot analyses were performed following SDS-PAGE under non-reducing (without 2-ME) and reducing (with 2-ME) conditions. (B) βHB inhibits H₂O₂-induced AMPKα oxidation in a Trx1-dependent manner. (C) βHB normalizes H₂O₂-induced AMPK inhibition in a Trx1-dependent manner. n = 6–10. Western blot analyses were performed with indicated antibodies. * p < 0.05 (C).