Figure 3.

Immunofluorescence detection of stemness and differentiation markers in iPSCs. Expression of OCT4, PAX6, SOX2, and NESTIN was assessed by immunofluorescence and confocal microscopy in both Eu-iPSC and T21-iPSC clones. T21-iPSC images are representative of the two trisomic clones. (A) Representative images show that both cell types are negative when stained with the PAX6 antibody (red) and positive with the anti-OCT4 antibody (green). The anti-OCT4 antibody stains the nucleoplasm with the exclusion of nucleoli. (B) Both cell types show a low level of signal, mostly in the perinuclear area, when stained with the NESTIN antibody (red). The cell nuclei are positive when cells are stained with the SOX2 antibody (green). Nuclei are also stained with Hoechst (blue). Bar = 10 μm.