Figure 6.

Low magnification acquisition of NESTIN and SOX2 positive NPCs. Euploid and trisomic NPCs were stained by double immunofluorescence with anti-NESTIN and anti-SOX2 antibodies at seven days of culture in differentiation medium. Fluorescent images were taken by the Thunder Imaging Systems. T21-NPC images are representative of the two trisomic clones. Eu-NPCs and T21-NPCs are positive when stained with either the NESTIN antibody (red) or the SOX2 antibody (green). Nuclei are also stained with DAPI (blue). Note the uniform staining of NESTIN and SOX2. Bar = 50 μm.