Figure 8.

Quantitative qRT-PCR analysis of neuronal, astroglial and oligodendroglial markers in NPCs at day 21 of neural induction. (A) The differentiated neuronal cell markers, MAP2 and TUBB3, were analyzed by qRT-PCR in euploid and trisomic NPCs, at day 21 of neural induction. An higher level of TUBB3 expression is observed in T21-NPCs. (B) The level of expression of GFAP, S100B, OLIG1 and OLIG2, was analyzed by qRT-PCR in euploid and trisomic NPCs. mRNA expression is normalized to a reference gene (ACTIN). T21-NPC values represent the average of the two trisomic clones used. Results are expressed as mean values ± SEM of at least four independent experiments for each cell line. * p < 0.05.