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. 2021 Jul 8;10(7):1095. doi: 10.3390/antiox10071095

Figure 2.

Figure 2

Decellularization of the striatal sections. (a) Non-decellularized (ECM) and decellularized (dECM) sections (250 µm thickness) from the striatum region of a C57/BL6 mouse brain stained with the nuclear dye DAPI (in blue) to assess the removal of cellular components; scale bar 100 µm. (b) The Alcian Blue staining of the ECM and dECM slices highlight the preservation of the glycosaminoglycans; scale bar 800 µm. (c) Detection of intracellular protein actin (left) and proteoglycanbrevican (right) by Western blot. It was possible to confirm the reduction of the cellular content and the preservation of ECM components on the dECM samples, respectively. Actin was present in high amounts in the solubilized non-decellularized ECM slices and in the lysate of the SH-SY5Y control cell line, whereas it was drastically reduced in the solubilized dECMs. (d) Immunostaining of collagen IV and fibronectin proteins in both ECM and dECM slices. The scale bar for ECM images is 1500 μm, while for the dECM collagen IV image it is 500 μm and for the dECM fibronectin image it is 1500 μm.