. 2021 Jun 24;10(7):578. doi: 10.3390/biology10070578

Table 3.

Hepatic qPCR analysis of microarray-identified transcripts, and comparison between the microarray and qPCR results.

Microarray Probe ^a	Transcript Name	qPCR RQ Values ^b			p-Value (qPCR) ^c	Fold-Change ^d
Microarray Probe ^a	Transcript Name	High ω3	Balanced	High ω6	p-Value (qPCR) ^c	Microarray	qPCR
N/A	htra1a	2.2 ± 0.41	1.9 ± 0.29	3.0 ± 0.65	0.25	N/A	1.34
C231R170	htra1b	6.0 ± 2.14	10.7 ± 3.56	22.4 ± 7.43	0.07	3.57	3.75
C103R052	p43	3.4 ± 0.66	2.9 ± 0.41	2.2 ± 0.47	0.24	−2.79	−1.59
C067R040	eif2a	5.2 ± 0.40	5.3 ± 0.74	3.5 ± 0.92	0.19	−3.13	−1.47
C253R093	eif4b1	8.8 ± 1.78	6.7 ± 1.43	5.5 ± 1.53	0.29	−3.23	−1.59
N/A	eif4b2	2.7 ± 0.30	2.7 ± 0.40	2.2 ± 0.30	0.55	N/A	−1.22
C060R108	mtco2	1.4 ± 0.10	1.2 ± 0.06	1.4 ± 0.14	0.43	−3.27	1.07
C159R112	lect2a	7.6 ± 2.58	4.0 ± 1.20	4.2 ± 0.96	0.38	−3.48	−1.79
N/A	lect2b	3.4 ± 0.74	3.7 ± 0.89	3.8 ± 0.61	0.96	N/A	1.12
C152R057	rpl18	2.0 ± 0.17	2.1 ± 0.25	2.2 ± 0.16	0.88	−4.37	1.08
C133R018	mef2d	1.9 ± 0.10 ^a,b	2.0 ± 0.11 ^a	1.5 ± 0.13 ^b	0.03	−4.54	−1.22
C065R088	helz2a	2.3 ± 0.32 ^a	1.6 ± 0.15 ^a,b	1.5 ± 0.14 ^b	0.04	−4.71	−1.49
N/A	helz2b	2.3 ± 0.21 ^a	1.4 ± 0.09 ^b	1.4 ± 0.11 ^b	0.002	N/A	−1.61
C002R106	itgb5	2.1 ± 0.23	1.9 ± 0.08	1.6 ± 0.08	0.13	−5.25	−1.34

^a Refers to the identity of the probe on the 44 K array. Transcripts with no probe ID are paralogues of microarray-identified transcripts. ^b Mean relative quantity (RQ) ± standard error (n = 6–8). RQ values were normalized to elongation factor 1 alpha-2 (eef1α-2) and 60S ribosomal protein 32 (rpl32), and calibrated to the lowest expressing individual for each gene of interest. Different letters indicate significant differences among treatments (General linear model followed by Tukey pairwise comparison). ^c p-values obtained in the qPCR study. Differences were considered statistically significant when p < 0.05. ^d Microarray and qPCR comparison of fold-changes (i.e., high ω6/high ω3). Down-regulated transcripts are negative values (−(1/fold-change)). qPCR fold-changes corresponding to GOI with significant differences between the high ω6 and high ω3 treatments are bolded.