Figure 1.

Nox4 is expressed in the proximal tubule cells and produces H₂O₂. (A): RNAscope in kidney. Left panel: +CTR: peptidylprolyl isomerase (B), −CTR: B. subtilis dihydrodipicolinate reductase. Middle: staining of Nox4 in cortex and medulla of WT and Nox4-/- mice. Right panel: higher magnification. Scale bar: 20 µm. B: RNAscope combined to IF shows Nox4 expression at the proximal tubule and not at the collecting tubule. (C): H₂O₂ measurement from renal tissue using Amplex red. * p < 0.05. n = 8 mice for each group.