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. 2021 Jul 9;9:619396. doi: 10.3389/fcell.2021.619396

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Copyright © 2021 Huang, Li, Wang, Wang, Zeng, Hu and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ER stress activation promotes migration and proliferative capacity of glioma cell lines in vitro. (A,B) The real-time quantitative PCR (qPCR) assay of PDIA4 and P4HB mRNA expression in LN229 and U87 cell lines after applying specific siRNA and negative control. (C,D) The western blot analysis of PDIA4 and P4HB protein expression in LN229 (C) and U87 (D) cell lines after applying specific siRNA and negative control. (E–H) Transwell migration assay of LN229 (E,F) and U87 (G,H) cell lines treated with specific siRNA and negative control. (I,J) CCK-8 assay displaying proliferation of LN229 (I) and U87 (J) cell lines treated with specific siRNA and negative control. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.