Skip to main content
. 2021 Jul 12;10(7):655. doi: 10.3390/biology10070655

Figure 6.

Figure 6

Overexpression of MCPIP1 inhibited cFLIP expression in TNF-α-treated cells. T-REx-293 cells with (A) wild-type hemagglutinin (HA)-MCPIP1 or (B) the HA-MCPIP1-D141N mutant (HA-D141N) were pretreated without or with 1 μg/mL of doxycycline (Dox) for 16 h, and then treated without or with 10 ng/mL TNF-α for 30~90 min. Total cell lysates were collected, and protein expressions were determined by Western blotting.