Figure 5.

The effect of Tofacitinib Citrate on IL-17A-induced retinal pJAK1 expression. C57BL/6J mice received IL-17A or vehicle (PBS) i.v. injection were treated with or without Tofacitinib Citrate twice. Retinae were collected 48 h later and processed for immunostaining of pJAK1. (A) Schematic of experimental design. (B) Representative images of pJAK1 expression in retinas of mice that received PBS, IL-17A, IL-17A + Tofacitinib Citrate or IL-17A + vehicle control; n ≥ 5 animals per group. (C) Quantification of pJAK1 integrated density. Two or three images from the central retina were quantified per animal. Values were normalized to PBS control on the same slide. Mean ± SD. ROUT outliers test 2% was used to remove outliers. Cleaned data were compared by using One-Way ANOVA with Tukey’s multiple comparisons test, * p < 0.05; ns: no statistical significance.