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. 2021 Jul 20;10(7):686. doi: 10.3390/biology10070686

Figure 5.

Figure 5

MYOC exerts an effect on the differentiation of C2C12 cells by regulation of CAV1 that affects the TGF-β pathway. (A). Change in MYOC, P-Smad2, and P-SMad4 expression after transfection of siRNA-sequence T3 for MYOC silencing. (B). Grayscale scan of MYOC in Figure A. (C). Grayscale scan of P-Smad2 in Figure A. (D). Grayscale scan of P-Smad4 in Figure A. (E). Western blotting of all indices in the co-transfection experiment control group and 3 experimental groups. (F). Grayscale scan of MYOC in Figure E. (G). Grayscale scan of CAV1 in Figure E. (H). Grayscale scan of MYH2 in Figure E. (I). Grayscale scan of MYOG in Figure E. (J). Grayscale scan of P-Smad2 in Figure E. (K). Grayscale scanning of P-Smad4 in Figure E. (L). Change in rate of fusion of myotubes after co-transfection, as measured by laser confocal microscopy. (M). Myotubule fusion rate in Figure L. (** p values < 0.01, * p values < 0.05) (n = 3).