Figure 1.

Production and characterization of rSur and rSur-FLIPr. (a) The purified proteins were examined by 10% tricine-PAGE with Coomassie Blue staining or examined by immunoblotting with anti-survivin and anti-FLIPr antibodies. Lane 1, marker; lane 2, rSur; lane 3, rSur-FLIPr. Binding ability of rSur-FLIPr to (b) mouse and (c) human Fcγ receptors. Various Fcγ receptor isoforms were coated on 96-well plates (0.5 μg/well). A serial dilution of biotin-conjugated rSur or rSur-FLIPr was added to each well and incubated at room temperature for 2 h. The binding proteins were detected by adding HRP-conjugated streptavidin. A substrate, TMB, was added for color development. The absorbance was measured with an ELISA reader at 450 nm. The data represent the means ± SE of the mean from two independent experiments.